Typical Testing Data/Standard Curve (for reference only)
Mouse VEGF ELISA Kit | VEGF elisa kit
VEGF (mouse) ELISA Kit
Gene Names
VEGFA; VEGF
Reactivity
Mouse
Synonyms
VEGF; VEGF (mouse) ELISA Kit; Vascular endothelial growth factor (mouse) ELISA kit; VEGF (mouse) ELISA kit; VEGF elisa kit
Reactivity
Mouse
Sequence Length
173
Application Notes
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples
Serum & plasma, Cell Culture Supernatants, Urine
Assay Type
Sandwich
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for murine VEGF.
Detection Range: 15.6 pg/ml - 1000 pg/ml.
This ELISA kit shows no species cross-reactivity.
Detection Range: 15.6 pg/ml - 1000 pg/ml.
This ELISA kit shows no species cross-reactivity.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VEGF elisa kit
Product Information: VEGF (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of murine VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.
Background: Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. MyBioSource's mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 2 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Detection of IgG Ab to TPO
Background: Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. MyBioSource's mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 2 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Detection of IgG Ab to TPO
Product Categories/Family for VEGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,368 Da
NCBI Official Full Name
VEGF, partial
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VEGF
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
Protein Family
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
UniProt Entry Name
VEGFA_PIG
Uniprot Description
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin ().
Research Articles on VEGF
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Product Notes
The Mouse VEGF vegfa (Catalog #AAA843481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843481 ELISA Kit recognizes Mouse VEGF. Quantitative protein detection, establishing normal range, validation of antibody array results. Researchers should empirically determine the suitability of the VEGF vegfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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