Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucin-20 (MUC20) Standard Curve.)
Mouse Mucin-20 (MUC20) ELISA Kit | MUC20 elisa kit
Mouse Mucin-20 (MUC20) ELISA Kit
Gene Names
Muc20; BC026367
Reactivity
Mouse
Synonyms
Mucin-20 (MUC20); Mouse Mucin-20 (MUC20) ELISA Kit; MUC20; FLJ14408; FLJ53153; KIAA1359; mucin 20; transmembrane mucin MUC20S; MUC20 elisa kit
Reactivity
Mouse
Specificity
Mouse Mucin-20 (MUC20) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MUC20. No significant cross-reactivity or interference between Mouse MUC20 and analogues was observed.
Sequence Length
688
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucin-20 (MUC20) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucin-20 (MUC20) Standard Curve.)
Related Product Information for MUC20 elisa kit
Description: This Mouse Mucin-20 (MUC20) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC20 in samples. An antibody specific for MUC20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MUC20 encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified.
Background: MUC20 encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mucin-20 isoform a
NCBI Official Synonym Full Names
mucin 20
NCBI Official Symbol
Muc20
NCBI Official Synonym Symbols
BC026367
NCBI Protein Information
mucin-20
UniProt Protein Name
Mucin-20
Protein Family
UniProt Gene Name
Muc20
UniProt Synonym Gene Names
MUC-20
UniProt Entry Name
MUC20_MOUSE
Uniprot Description
MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.
Cellular Component: cell projection; membrane; extracellular region; basal plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; protein homooligomerization
Research Articles on MUC20
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Product Notes
The Mouse MUC20 muc20 (Catalog #AAA9717505) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717505 ELISA Kit recognizes Mouse MUC20. It is sometimes possible for the material contained within the vial of "Mucin-20 (MUC20), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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