Human Prestin (PRES) ELISA Kit | PRES elisa kit

Human Prestin (PRES) ELISA Kit

Cat. Num. AAA167508

• Gene Names: Slc26a5; Pres; prestin
• Reactivity: Human
• Synonyms: Prestin (PRES); Human Prestin (PRES) ELISA Kit; PRES elisa kit

• Reactivity: Human
• Sequence Length: 744

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 10-3000pg/ml
• Sensitivity: 4.87pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRES elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Prestin (also known as PRES) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PRES antibody. PRES present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRES Antibody is added and binds to PRES in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRES antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PRES. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 13540646
• NCBI GeneID: 83819
• NCBI Accession #: NP_110467.1
• NCBI GenBank Nucleotide #: NM_030840.1
• UniProt Accession #: Q9EPH0; Q9ERC6
• Molecular Weight: 81,279 Da
• NCBI Official Full Name: prestin
• NCBI Official Synonym Full Names: solute carrier family 26 member 5
• NCBI Official Symbol: Slc26a5
• NCBI Official Synonym Symbols: Pres; prestin
• NCBI Protein Information: prestin
• UniProt Protein Name: Prestin
• UniProt Gene Name: Slc26a5
• UniProt Synonym Gene Names: Pres

NCBI Description

molecular motor protein; involved in the electromechanical properties of hearing [RGD, Feb 2006]

Uniprot Description

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site ().