Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-3A (MUC3A) Standard Curve.)
Human Mucin-3A (MUC3A) ELISA Kit | MUC3A elisa kit
Human Mucin-3A (MUC3A) ELISA Kit
Gene Names
MUC3A; MUC3; MUC-3A
Reactivity
Human
Synonyms
Mucin-3A (MUC3A); Human Mucin-3A (MUC3A) ELISA Kit; MUC3A; MUC3; mucin; mucin 3; intestinal; mucin 3A; MUC3A elisa kit
Reactivity
Human
Specificity
Human Mucin-3A (MUC3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC3A. No significant cross-reactivity or interference between Human MUC3A and analogues was observed.
Sequence Length
3323
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-3A (MUC3A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-3A (MUC3A) Standard Curve.)
Related Product Information for MUC3A elisa kit
Description: This Human Mucin-3A (MUC3A) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC3A in samples. An antibody specific for MUC3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation.
Background: The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mucin-3A
NCBI Official Synonym Full Names
mucin 3A, cell surface associated
NCBI Official Symbol
MUC3A
NCBI Official Synonym Symbols
MUC3; MUC-3A
NCBI Protein Information
mucin-3A
UniProt Protein Name
Mucin-3A
Protein Family
UniProt Gene Name
MUC3A
UniProt Synonym Gene Names
MUC-3ACurated
UniProt Entry Name
MUC3A_HUMAN
NCBI Description
The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 [PubMed 7778880]).[supplied by OMIM, Aug 2008]
Uniprot Description
MUC3A: Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: Golgi lumen
Biological Process: O-glycan processing
Disease: Inflammatory Bowel Disease 11
Research Articles on MUC3A
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Product Notes
The Human MUC3A muc3a (Catalog #AAA9715119) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715119 ELISA Kit recognizes Human MUC3A. It is sometimes possible for the material contained within the vial of "Mucin-3A (MUC3A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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