Human Mucin-13 (MUC13) ELISA Kit | MUC13 elisa kit

Human Mucin-13 (MUC13) ELISA Kit

Cat. Num. AAA9715127

• Gene Names: MUC13; DRCC1; MUC-13
• Reactivity: Human
• Synonyms: Mucin-13 (MUC13); Human Mucin-13 (MUC13) ELISA Kit; MUC13; DRCC1; FLJ20063; down-regulated in colon cancer 1; mucin 13; epithelial transmembrane; MUC13 elisa kit

• Reactivity: Human
• Specificity: Human Mucin-13 (MUC13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC13. No significant cross-reactivity or interference between Human MUC13 and analogues was observed.
• Sequence Length: 512

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Mucin-13 (MUC13) Standard Curve.)

Related Product Information for MUC13 elisa kit

Description: This Human Mucin-13 (MUC13) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC13 in samples. An antibody specific for MUC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 308736985
• NCBI GeneID: 56667
• NCBI Accession #: NP_149038.3
• NCBI GenBank Nucleotide #: NP_149038.3
• UniProt Accession #: Q9H3R2
• NCBI Official Full Name: mucin-13
• NCBI Official Synonym Full Names: mucin 13, cell surface associated
• NCBI Official Symbol: MUC13
• NCBI Official Synonym Symbols: DRCC1; MUC-13
• NCBI Protein Information: mucin-13
• UniProt Protein Name: Mucin-13
• Protein Family: Mucin
• UniProt Gene Name: MUC13
• UniProt Synonym Gene Names: DRCC1; RECC; MUC-13
• UniProt Entry Name: MUC13_HUMAN

NCBI Description

Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 [PubMed 11278439]).[supplied by OMIM, Jul 2008]

Uniprot Description

MUC13: Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: extracellular space; Golgi lumen; apical plasma membrane; integral to membrane; cytosol

Molecular Function: protein homodimerization activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; post-translational protein modification