Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-21 (MUC21) Standard Curve.)
Human Mucin-21 (MUC21) ELISA Kit | MUC21 elisa kit
Human Mucin-21 (MUC21) ELISA Kit
Gene Names
MUC21; MUC-21; KMQK697; C6orf205
Reactivity
Human
Synonyms
Mucin-21 (MUC21); Human Mucin-21 (MUC21) ELISA Kit; MUC21; DAAP-223I5.2; C6orf205; KMQK697; MGC125609; bCX31G15.2; epiglycanin; mucin 21; MUC21 elisa kit
Reactivity
Human
Specificity
Human Mucin-21 (MUC21) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC21. No significant cross-reactivity or interference between Human MUC21 and analogues was observed.
Sequence Length
566
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-21 (MUC21) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mucin-21 (MUC21) Standard Curve.)
Related Product Information for MUC21 elisa kit
Description: This Human Mucin-21 (MUC21) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC21 in samples. An antibody specific for MUC21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MUC21 encodes a large membrane-bound glycoprotein which is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. The encoded protein contains an N-terminal signal sequence, an extracellular mucin domain, a stem domain, a transmembrane domain, and a C-terminal cytoplasmic tail domain. The mucin domain contains O-glycosylation sites and is polymorphic with isoforms containing a variable number of nonidentical proline-, threonine-, and serine-rich tandem repeats of 15 amino acids each. The aberrent expression of MUC21 is associated with lung adenocarcinoma.
Background: MUC21 encodes a large membrane-bound glycoprotein which is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. The encoded protein contains an N-terminal signal sequence, an extracellular mucin domain, a stem domain, a transmembrane domain, and a C-terminal cytoplasmic tail domain. The mucin domain contains O-glycosylation sites and is polymorphic with isoforms containing a variable number of nonidentical proline-, threonine-, and serine-rich tandem repeats of 15 amino acids each. The aberrent expression of MUC21 is associated with lung adenocarcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mucin-21 isoform 1
NCBI Official Synonym Full Names
mucin 21, cell surface associated
NCBI Official Symbol
MUC21
NCBI Official Synonym Symbols
MUC-21; KMQK697; C6orf205
NCBI Protein Information
mucin-21
UniProt Protein Name
Mucin-21
Protein Family
UniProt Gene Name
MUC21
UniProt Synonym Gene Names
C6orf205; MUC-21
NCBI Description
This gene encodes a large membrane-bound glycoprotein which is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. The encoded protein contains an N-terminal signal sequence, an extracellular mucin domain, a stem domain, a transmembrane domain, and a C-terminal cytoplasmic tail domain. The mucin domain contains O-glycosylation sites and is polymorphic with isoforms containing a variable number of nonidentical proline-, threonine-, and serine-rich tandem repeats of 15 amino acids each. The aberrent expression of this gene is associated with lung adenocarcinoma. [provided by RefSeq, May 2017]
Research Articles on MUC21
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Product Notes
The Human MUC21 muc21 (Catalog #AAA9715120) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715120 ELISA Kit recognizes Human MUC21. It is sometimes possible for the material contained within the vial of "Mucin-21 (MUC21), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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