Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanotransferrin (MFI2) Standard Curve.)
Mouse Melanotransferrin (MFI2) ELISA Kit | MFI2 elisa kit
Mouse Melanotransferrin (MFI2) ELISA Kit
Gene Names
Meltf; MTf; Mfi2; CD228
Reactivity
Mouse
Synonyms
Melanotransferrin (MFI2); Mouse Melanotransferrin (MFI2) ELISA Kit; MFI2; CD228; FLJ38863; MAP97; MGC4856; MTF1; melanoma-associated antigen p97; isoform 2; melanotransferrin; membrane-bound transferrin-like protein; MFI2 elisa kit
Reactivity
Mouse
Specificity
Mouse Melanotransferrin (MFI2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MFI2. No significant cross-reactivity or interference between Mouse MFI2 and analogues was observed.
Sequence Length
738
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanotransferrin (MFI2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melanotransferrin (MFI2) Standard Curve.)
Related Product Information for MFI2 elisa kit
Description: This Mouse Melanotransferrin (MFI2) ELISA Kit employs a two-site sandwich ELISA to quantitate MFI2 in samples. An antibody specific for MFI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by MELTF is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. MELTF resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
Background: The protein encoded by MELTF is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. MELTF resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
melanotransferrin
NCBI Official Synonym Full Names
melanotransferrin
NCBI Official Symbol
Meltf
NCBI Official Synonym Symbols
MTf; Mfi2; CD228
NCBI Protein Information
melanotransferrin
UniProt Protein Name
Melanotransferrin
UniProt Gene Name
Mfi2
UniProt Synonym Gene Names
Mtf; MTf
UniProt Entry Name
TRFM_MOUSE
Research Articles on MFI2
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Product Notes
The Mouse MFI2 mfi2 (Catalog #AAA9717651) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717651 ELISA Kit recognizes Mouse MFI2. It is sometimes possible for the material contained within the vial of "Melanotransferrin (MFI2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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