Rabbit anti-Human Mapk15 Polyclonal Antibody | anti-MAPK15 antibody

Mapk15, ID (Mapk15, Erk7, ERK8, Mitogen-activated protein kinase 15, Extracellular signal-regulated kinase 8)

Cat. Num. AAA646868

• Gene Names: MAPK15; ERK7; ERK8
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Mapk15; Polyclonal Antibody; ID (Mapk15; Erk7; ERK8; Mitogen-activated protein kinase 15; Extracellular signal-regulated kinase 8); Anti -Mapk15; anti-MAPK15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAPK15.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MAPK15 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:5000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human MAPK15.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-MAPK15 antibody

ERK8 is a MAPK type protein kinase that is activated by a Src-dependent signaling pathway. The protein contains two SH3-binding motifs in its C-terminal region and has been shown to associate with the c-Src SH3 domain in vitro and co-immunoprecipitate with c-Src in vivo.

Product Categories/Family for anti-MAPK15 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 95147356
• NCBI GeneID: 225689
• NCBI Accession #: NP_620590.2
• NCBI GenBank Nucleotide #: NM_139021.2
• UniProt Accession #: Q8TD08; Q2TCF9; Q8N362
• Molecular Weight: 59,832 Da
• NCBI Official Full Name: mitogen-activated protein kinase 15
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 15
• NCBI Official Symbol: MAPK15
• NCBI Official Synonym Symbols: ERK7; ERK8
• NCBI Protein Information: mitogen-activated protein kinase 15; ERK-7; ERK-8; MAPK 15; MAP kinase 15; extracellular regulated kinase 8 delta; extracellular signal regulated kinase 8; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 15
• UniProt Gene Name: MAPK15
• UniProt Synonym Gene Names: ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
• UniProt Entry Name: MK15_HUMAN

Uniprot Description

Function: In vitro, phosphorylates MBP. Ref.1 Ref.2

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1

By similarity.

Subunit structure: Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. Ref.1 Ref.2 Ref.4

Tissue specificity: Widely expressed with a maximal expression in lung and kidney. Ref.1 Ref.2

Domain: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation

By similarity.The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. Ref.1Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.