Human Melanotransferrin (MFI2) ELISA Kit | MFI2 elisa kit

Human Melanotransferrin (MFI2) ELISA Kit

Cat. Num. AAA2703577

• Gene Names: MELTF; MTf; MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Synonyms: Melanotransferrin (MFI2); Human Melanotransferrin (MFI2) ELISA Kit; CD228; MAP97; MTF1; Antigen P97(Melanoma Associated); Membrane-Bound Transferrin-Like Protein; MFI2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Melanotransferrin (MFI2).; No significant cross-reactivity or interference between Melanotransferrin (MFI2) and analogues was observed.
• Sequence Length: 302

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 12.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanotransferrin (MFI2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanotransferrin (MFI2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFI2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanotransferrin (MFI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanotransferrin (MFI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanotransferrin (MFI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanotransferrin (MFI2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16163666
• NCBI GeneID: 4241
• NCBI Accession #: NP_201573.1
• NCBI GenBank Nucleotide #: NM_033316.3
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 32,723 Da
• NCBI Official Full Name: melanotransferrin isoform 2
• NCBI Official Synonym Full Names: melanotransferrin
• NCBI Official Symbol: MELTF
• NCBI Official Synonym Symbols: MTf; MFI2; MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin
• UniProt Protein Name: Melanotransferrin
• UniProt Gene Name: MELTF

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.