Typical Testing Data
Human Melanotransferrin ELISA Kit | MFI2 elisa kit
Human Melanotransferrin ELISA Kit
Reactivity
Human
Synonyms
Melanotransferrin; Human Melanotransferrin ELISA Kit; MFI2/CD228/MAP97/MFI2/MTF/p97/antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5/CD228/CD228 antigen/FLJ38863/MAP97Melanoma-associated antigen p97/melanotransferrin/membrane-bound transferrin-like protein/MGC4856/MTF1; MFI2 elisa kit
Reactivity
Human
Sequence Length
738
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data
Typical Testing Data
Typical Standard Curve
Typical Standard Curve
Related Product Information for MFI2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MFI2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MFI2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MFI2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman MFI2 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,723 Da
NCBI Official Full Name
melanotransferrin
UniProt Protein Name
Melanotransferrin
Protein Family
UniProt Gene Name
MFI2
UniProt Synonym Gene Names
MAP97
UniProt Entry Name
TRFM_HUMAN
Uniprot Description
MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 3q28-q29
Cellular Component: anchored to plasma membrane; cell surface; extracellular space; integral to plasma membrane
Molecular Function: iron ion binding; protein binding
Biological Process: iron ion homeostasis; regulation of cell growth; regulation of cell proliferation
Similar Products
Product Notes
The Human MFI2 mfi2 (Catalog #AAA761797) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA761797 ELISA Kit recognizes Human MFI2. It is sometimes possible for the material contained within the vial of "Melanotransferrin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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