Typical Testing Data/Standard Curve (for reference only)
(Rat IL-2 PicoKine ELISA Kit standard curve )
Rat IL-2 ELISA Kit | IL2 elisa kit
Rat IL-2 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
IL-2; Rat IL-2 PicoKine ELISA Kit; Interleukin-2; interleukin 2; Aldesleukin; IL 2; IL2; IL2_HUMAN; Interleukin 2; Interleukin2; Involved in regulation of T cell clonal expansion; Lymphokine; T cell growth factor; T-cell growth factor; TCGF; IL2 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat IL-2
Sequence Length
155
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat IL-2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat IL-2 PicoKine ELISA Kit standard curve )
Related Product Information for IL2 elisa kit
Principle of the assay: rat IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-2 amount of sample captured in plate.
Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3
Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,633 Da
NCBI Official Full Name
Interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
Il2
NCBI Protein Information
interleukin-2; IL-2; TCGF; T-cell growth factor
UniProt Protein Name
Interleukin-2
Protein Family
UniProt Gene Name
Il2
UniProt Synonym Gene Names
Il-2; IL-2; TCGF
UniProt Entry Name
IL2_RAT
NCBI Description
cytokine produced by T-cells in response to antigen or mitogen stimulation [RGD, Feb 2006]
Uniprot Description
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Research Articles on IL2
Similar Products
Product Notes
The Rat IL2 il2 (Catalog #AAA175774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175774 ELISA Kit recognizes Rat IL2. It is sometimes possible for the material contained within the vial of "IL-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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