IL-17F elisa kit

Human IL-17F PicoKine ELISA Kit

Cat. Num. AAA1751542

• Gene Names: IL17F; ML1; ML-1; CANDF6; IL-17F
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: IL-17F; Human IL-17F PicoKine ELISA Kit; Human IL-17F; Interleukin-17F; Cytokine ML-1; IL17F; Interleukin 17F; IL-17F elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human IL17A
• Sequence Length: 163

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-17F elisa kit

Principle of the Assay: The Picokine Human IL17A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL17A with a 96-well strip plate that is pre-coated with antibody specific for IL17A. The detection antibody is a biotinylated antibody specific for IL17A. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human IL17A with immunogen: Expression system for standard: E.coli; Immunogen sequence: I20-A155. The kit is analytically validated with ready to use reagents. To measure Human IL17A, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human IL17A in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL17A in the sample!!Background/Introduction: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17-to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 16418375
• NCBI GeneID: 112744
• NCBI Accession #: NP_443104.1
• NCBI GenBank Nucleotide #: NM_052872.3
• UniProt Accession #: Q96PD4; Q6NSI0; Q7Z6P4; Q96PI8; Q9NUE6
• Molecular Weight: 18,045 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: IL17F
• NCBI Official Synonym Symbols: ML1; ML-1; CANDF6; IL-17F
• NCBI Protein Information: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• UniProt Gene Name: IL17F
• UniProt Synonym Gene Names: IL-17F

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation ().