Rat IL-13 ELISA Kit | IL13 elisa kit

Rat IL-13 PicoKine ELISA Kit

Cat. Num. AAA175932

• Reactivity: Rat
• Synonyms: IL-13; Rat IL-13 PicoKine ELISA Kit; Interleukin-13; interleukin 13; ALRH; BHR 1; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; Il13; IL13_HUMAN; Interleukin 13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; P600; T-cell activation protein P600; IL13 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat IL13
• Sequence Length: 131

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL13 elisa kit

Principle of the Assay: The Picokine™ Rat IL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat IL13 with a 96-well strip plate that is pre-coated with antibody specific for IL13. The detection antibody is a biotinylated antibody specific for IL13. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat IL13 with immunogen: Expression system for standard: E.coli; Immunogen sequence: T19-H131. The kit is analytically validated with ready to use reagents. To measure Rat IL13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat IL13 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat IL13 in the sample.!!Background/Introduction: Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.

NCBI and Uniprot Product Information

• NCBI GI #: 1170528
• NCBI GeneID: 116553
• UniProt Accession #: P42203
• Molecular Weight: 14,093 Da
• NCBI Official Full Name: Interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: Il13
• NCBI Protein Information: interleukin-13; IL-13; T-cell activation protein P600
• UniProt Protein Name: Interleukin-13
• Protein Family: Interleukin
• UniProt Gene Name: Il13
• UniProt Synonym Gene Names: Il-13; IL-13
• UniProt Entry Name: IL13_RAT

NCBI Description

cytokine; involved in inflammatory and immune responses [RGD, Feb 2006]

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; positive regulation of mast cell degranulation; response to lipopolysaccharide; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; response to mechanical stimulus; positive regulation of B cell proliferation; immune response; positive regulation of ion transport; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; inflammatory response; positive regulation of macrophage activation