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Western Blot (WB) (Western Blot; Sample: Human Lung lysate Primary Ab: 2?g/ml Mouse Anti-Human SOD3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Mouse anti-Human Superoxide Dismutase 3 (SOD3) Monoclonal Antibody | anti-SOD3 antibody

Monoclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)

Gene Names
SOD3; EC-SOD
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G Affinity Chromatography
Synonyms
Superoxide Dismutase 3 (SOD3); Monoclonal Antibody; Monoclonal Antibody to Superoxide Dismutase 3; Extracellular (SOD3); SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn; anti-SOD3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C3
Specificity
The antibody is a mouse monoclonal antibody raised against SOD3. It has been selected for its ability to recognize SOD3 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Trp19~Ala240
Applicable Applications for anti-SOD3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
Immunogen Sequence
Trp19~Ala240
Cross Reactivity
Human
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Human Lung lysate Primary Ab: 2?g/ml Mouse Anti-Human SOD3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot; Sample: Human Lung lysate Primary Ab: 2?g/ml Mouse Anti-Human SOD3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20ug/ml Mouse Anti-Human SOD3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Product Categories/Family for anti-SOD3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
240
NCBI Official Full Name
Extracellular superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 3, extracellular
NCBI Official Symbol
SOD3
NCBI Official Synonym Symbols
EC-SOD
NCBI Protein Information
extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]
UniProt Protein Name
Extracellular superoxide dismutase [Cu-Zn]
UniProt Gene Name
SOD3
UniProt Synonym Gene Names
EC-SOD
UniProt Entry Name
SODE_HUMAN

NCBI Description

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: EC 1.15.1.1; Secreted, signal peptide; Oxidoreductase; Secreted

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; response to copper ion; removal of superoxide radicals; response to hypoxia

Research Articles on SOD3

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Product Notes

The SOD3 sod3 (Catalog #AAA2111521) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase 3 (SOD3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the SOD3 sod3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Trp19~Ala2 40. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 3 (SOD3), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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