Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit | FGF21 elisa kit

Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit

Cat. Num. AAA2700587

• Reactivity: Mouse
• Synonyms: Fibroblast Growth Factor 21 (FGF21); Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit; FGF21 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21).; No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
• Sequence Length: 209

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 12.5-200pg/mL
• Sensitivity: < 5.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF21 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 21 (FGF21) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 21 (FGF21) and unlabeled Fibroblast Growth Factor 21 (FGF21) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 21 (FGF21). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 21 (FGF21) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 21 (FGF21) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 9506597
• NCBI GeneID: 26291
• NCBI Accession #: NP_061986.1
• NCBI GenBank Nucleotide #: NM_019113.3
• UniProt Accession #: Q9NSA1; Q8N683
• Molecular Weight: 22,300 Da
• NCBI Official Full Name: fibroblast growth factor 21
• NCBI Official Synonym Full Names: fibroblast growth factor 21
• NCBI Official Symbol: FGF21
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF21
• UniProt Synonym Gene Names: FGF-21

NCBI Description

Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell-cell signaling; endoplasmic reticulum unfolded protein response; positive regulation of cell proliferation; positive regulation of glucose import; positive regulation of low-density lipoprotein particle receptor biosynthetic process; response to activity; response to nutrient levels; signal transduction