Human Fibroblast Growth Factor 13 (FGF13) ELISA Kit | FGF13 elisa kit

Human Fibroblast Growth Factor 13 (FGF13) ELISA Kit

Cat. Num. AAA2700586

• Gene Names: Fgf13; FGF-13
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 13 (FGF13); Human Fibroblast Growth Factor 13 (FGF13) ELISA Kit; FHF2; Fibroblast Growth Factor Homologous Factor 2; FGF13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13).; No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
• Sequence Length: 192

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 31.2-500pg/mL
• Sensitivity: < 13.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF13 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 13 (FGF13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 13 (FGF13) and unlabeled Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 13 (FGF13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 13 (FGF13) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 16758168
• NCBI GeneID: 84488
• NCBI Accession #: NP_445880.1
• NCBI GenBank Nucleotide #: NM_053428.1
• Molecular Weight: 21,605 Da
• NCBI Official Full Name: fibroblast growth factor 13
• NCBI Official Synonym Full Names: fibroblast growth factor 13
• NCBI Official Symbol: Fgf13
• NCBI Official Synonym Symbols: FGF-13
• NCBI Protein Information: fibroblast growth factor 13
• UniProt Protein Name: Fibroblast growth factor 13
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf13
• UniProt Synonym Gene Names: FGF-13

NCBI Description

may play a role in differentiation of cortical neurons [RGD, Feb 2006]

Uniprot Description

FGF13: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus. Belongs to the heparin-binding growth factors family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Nucleolus

Chromosomal Location of Human Ortholog: Xq36

Cellular Component: axon; cytoplasm; cytosol; dendrite; extracellular region; filopodium; growth cone; lateral plasma membrane; microtubule; neuron projection; nucleus; plasma membrane

Molecular Function: beta-tubulin binding; growth factor activity; microtubule binding; protein kinase activator activity; sodium channel regulator activity

Biological Process: activation of protein kinase activity; apoptosis; cell proliferation; cerebral cortex cell migration; establishment of neuroblast polarity; hippocampus development; learning; MAPK cascade; memory; microtubule polymerization; negative regulation of collateral sprouting; negative regulation of microtubule depolymerization; neuron migration; sodium ion transport