Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL2 expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with PE-conjugated anti-Human IL2 and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Mouse anti-Human IL2 Monoclonal Antibody | anti-IL2 antibody
Anti-IL2 Antibody (PE), Mouse Monoclonal
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL2 expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with PE-conjugated anti-Human IL2 and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL2 expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with PE-conjugated anti-Human IL2 and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Interleukin-2/IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2/IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2/IL-2 and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2/IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2/IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2/IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2/IL-2 in HIV therapy is ineffective.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Uniprot Description
IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.
Protein type: Oncoprotein; Cytokine; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q26-q27
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; glycosphingolipid binding; carbohydrate binding; kappa-type opioid receptor binding
Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; negative regulation of protein amino acid phosphorylation; protein kinase C activation; positive regulation of cell proliferation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis
Research Articles on IL2
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Product Notes
The IL2 il2 (Catalog #AAA8105903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL2 Antibody (PE), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the IL2 il2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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