Mouse Complement Component 5a (C5a) ELISA Kit | C5a elisa kit

Mouse Complement Component 5a (C5a)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709608

• Gene Names: Hc; C5; He; C5a
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Complement Component 5a (C5a); Mouse Complement Component 5a (C5a)(Sandwich ELISA) ELISA Kit; Mini Samples ELISA Kit for Complement Component 5a (C5a); C5a/Complement Component 5a; C5a elisa kit

• Reactivity: Mouse
• Specificity: Reactive with Mouse Complement Component 5a (C5a); This assay has high sensitivity and excellent specificity for detection of Mini Samples Complement Component 5a (C5a). No significant cross-reactivity or interference between Mini Samples Complement Component 5a (C5a) and analogues was observed.

• Applicable Applications for C5a elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA-Mini Samples
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.59ng/mL
• Assay Length: 3 h
• Sample Size: 15-25ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Complement Component 5a (C5a) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C; 3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 20ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 1 (PON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 1 (PON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 1 (PON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Paraoxonase 1 (PON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for C5a elisa kit

Metabolic pathway; Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 6754164
• NCBI GeneID: 15139
• NCBI Accession #: NP_034536.1
• NCBI GenBank Nucleotide #: NM_010406.2
• UniProt Accession #: P06684; A2AS36
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: hemolytic complement
• NCBI Official Symbol: Hc
• NCBI Official Synonym Symbols: C5; He; C5a
• NCBI Protein Information: complement C5
• UniProt Protein Name: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: Hc
• UniProt Entry Name: CO5_MOUSE

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: endopeptidase inhibitor activity

Biological Process: positive regulation of angiogenesis; in utero embryonic development; immune system process; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway