Mouse Complement C5 ELISA Kit | C5 elisa kit

Mouse Complement C5 ELISA Kit

Cat. Num. AAA824906

• Gene Names: Hc; C5; He; C5a
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Complement C5; Mouse Complement C5 ELISA Kit; CPAMD4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Complement C5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Complement C5 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 1680

• Applicable Applications for C5 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C5 elisa kit

Principle of the Assay: The MyBioSource' Mouse Complement C5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Complement C5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Complement C5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Complement C5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Complement C5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Complement C5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 6754164
• NCBI GeneID: 15139
• NCBI Accession #: NP_034536.1
• NCBI GenBank Nucleotide #: NM_010406.2
• UniProt Accession #: P06684; A2AS36
• Molecular Weight: 188,878 Da
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: hemolytic complement
• NCBI Official Symbol: Hc
• NCBI Official Synonym Symbols: C5; He; C5a
• NCBI Protein Information: complement C5
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: Hc
• UniProt Entry Name: CO5_MOUSE

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: endopeptidase inhibitor activity

Biological Process: positive regulation of angiogenesis; immune system process; in utero embryonic development; cytolysis; complement activation, alternative pathway; innate immune response; inflammatory response; complement activation, classical pathway