Western Blot (WB)
Rabbit PDE1B Polyclonal Antibody | anti-PDE1B antibody
PDE1B Antibody
Gene Names
Pde1b; Pde1b1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
PDE1B; Polyclonal Antibody; PDE1B Antibody; 5''-cyclic nucleotide phosphodiesterase 1B; 63 kDa Cam PDE; Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B; PDE1B1; Cam PDE 1B; PDES 1B; Phosphodiesterase 1B; Phosphodiesterase 1B calmodulin dependent antibody; anti-PDE1B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
This antibody does not cross-react with PDE1A or PDE1C or any known PDE family m ember. This antibody will label all PDE1B variants.
Form/Format
Affinity Purified
Concentration
0.58ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
479
Applicable Applications for anti-PDE1B antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Confocal Microscopy||1:50-1:200
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:50-1:200
Immunofluorescence: 1:50-1:200
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: 1:200
Western Blot: 1:500-1:1,000.
This antibody labels multiple bands in the 65-72 kDa range all believed to be various PDE1B variants.
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:50-1:200
Immunofluorescence: 1:50-1:200
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: 1:200
Western Blot: 1:500-1:1,000.
This antibody labels multiple bands in the 65-72 kDa range all believed to be various PDE1B variants.
Immunogen
Synthetic peptide corresponding to unique amino acid sequence on PDE1B gene.
Molecular Function
Calmodulin-binding, cAMP, cGMP, Metal-binding
Structure
Homodimer
Subcellular Location
Cytoplasm
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Related Product Information for anti-PDE1B antibody
Affinity Purified Pan Phosphodiesterase 1B Antibody
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
Product Categories/Family for anti-PDE1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted: 65 kDa.
NCBI Official Full Name
phosphodiesterase 1B, Ca2+-calmodulin dependent, isoform CRA_b, partial
NCBI Official Synonym Full Names
phosphodiesterase 1B, Ca2+-calmodulin dependent
NCBI Official Symbol
Pde1b
NCBI Official Synonym Symbols
Pde1b1
NCBI Protein Information
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
UniProt Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
UniProt Gene Name
Pde1b
UniProt Synonym Gene Names
Pde1b1; Cam-PDE 1B
UniProt Entry Name
PDE1B_MOUSE
Uniprot Description
PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.17; Phosphodiesterase; Apoptosis; Nucleotide Metabolism - purine
Cellular Component: cell soma; cytoplasm
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin binding; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity
Biological Process: cAMP catabolic process; cGMP catabolic process; locomotory behavior; regulation of dopamine metabolic process; regulation of neurotransmitter levels; response to amphetamine; serotonin metabolic process; signal transduction; visual learning
Research Articles on PDE1B
Similar Products
Product Notes
The PDE1B pde1b (Catalog #AAA540576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDE1B can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Confocal Microscopy||1:50-1:200 Dot Blot: 1:10,000 ELISA: 1:10,000 Immunocytochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200 Immunohistochemistry: 1:50-1:200 Immunoprecipitation: 1:200 Western Blot: 1:500-1:1,000. This antibody labels multiple bands in the 65-72 kDa range all believed to be various PDE1B variants. Researchers should empirically determine the suitability of the PDE1B pde1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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