Human Gastrin ELISA Kit | GAST elisa kit

Human Gastrin ELISA Kit

Cat. Num. AAA762206

• Gene Names: GAST; GAS
• Reactivity: Human
• Synonyms: Gastrin; Human Gastrin ELISA Kit; GT (Gastrin)/GAST/GAS; GAST elisa kit

• Reactivity: Human
• Sequence Length: 101

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.313-20pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GAST elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Gastrin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Gastrin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Gastrin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Gastrin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503923
• NCBI GeneID: 2520
• NCBI Accession #: NP_000796.1
• NCBI GenBank Nucleotide #: NM_000805.4
• Molecular Weight: 11,394 Da
• NCBI Official Full Name: gastrin preproprotein
• NCBI Official Synonym Full Names: gastrin
• NCBI Official Symbol: GAST
• NCBI Official Synonym Symbols: GAS
• NCBI Protein Information: gastrin
• UniProt Protein Name: Gastrin
• Protein Family: Gastrin
• UniProt Gene Name: GAST
• UniProt Synonym Gene Names: GAS; G52; G34; G17; G14; G6
• UniProt Entry Name: GAST_HUMAN

NCBI Description

Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]

Uniprot Description

gastrin: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular region; extracellular space

Molecular Function: hormone activity; protein binding

Biological Process: G-protein coupled receptor protein signaling pathway; response to food; signal transduction