Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL28 PicoKine ELISA Kit standard curve )
Mouse CCL28 ELISA Kit | CCL28 elisa kit
Mouse CCL28 PicoKine ELISA Kit
Gene Names
Ccl28; MEC; CCK1; Scya28
Reactivity
Mouse
Synonyms
CCL28; Mouse CCL28 PicoKine ELISA Kit; C-C motif chemokine 28; chemokine (C-C motif) ligand 28; CCK 1; CCK1; CCL 28; Chemokine (C-C motif) ligand 28; MEC; MGC71902; Mucosae associated epithelial chemokine; OTTHUMP0000022162; SCYA28; Small inducible cytokine A28; Small inducible cytokine A28 [Precursor]; Small inducible cytokine subfamily A (Cys-Cys); member 28; CCL28 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CCL28
Sequence Length
130
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL28 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL28 PicoKine ELISA Kit standard curve )
Related Product Information for CCL28 elisa kit
Principle of the assay: mouse CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL28 has been precoated onto 96-well plates. Standards (E.coli, I23-R130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL28 amount of sample captured in plate.
Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,570 Da
NCBI Official Full Name
C-C motif chemokine 28
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 28
NCBI Official Symbol
Ccl28
NCBI Official Synonym Symbols
MEC; CCK1; Scya28
NCBI Protein Information
C-C motif chemokine 28; small inducible cytokine A28; small-inducible cytokine A28
UniProt Protein Name
C-C motif chemokine 28
Protein Family
UniProt Gene Name
Ccl28
UniProt Synonym Gene Names
Scya28
UniProt Entry Name
CCL28_MOUSE
Uniprot Description
CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted
Cellular Component: extracellular space; cell; extracellular region; cytosol
Molecular Function: chemokine activity; cytokine activity
Biological Process: elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion; chemotaxis
Research Articles on CCL28
Similar Products
Product Notes
The Mouse CCL28 ccl28 (Catalog #AAA177311) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177311 ELISA Kit recognizes Mouse CCL28. It is sometimes possible for the material contained within the vial of "CCL28, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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