Human CCL28 ELISA Kit | CCL28 elisa kit

Human CCL28 ELISA Kit

Cat. Num. AAA8248337

• Gene Names: CCL28; MEC; CCK1; SCYA28
• Reactivity: Human
• Synonyms: CCL28; Human CCL28 ELISA Kit; SCYA28; C-C motif chemokine 28; Mucosae-associated epithelial chemokine; MEC; Protein CCK1; Small-inducible cytokine A28; CCL28 elisa kit

• Reactivity: Human
• Specificity: The Human CCL28 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CCL28 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 127

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL28 elisa kit

Principle of the Assay: The Human CCL28 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

NCBI and Uniprot Product Information

• NCBI GI #: 687569447
• NCBI GeneID: 56477
• NCBI Accession #: NP_001288802.1
• NCBI GenBank Nucleotide #: NM_001301873.1
• UniProt Accession #: Q9NRJ3; D7RIE7
• Molecular Weight: 12,375 Da
• NCBI Official Full Name: C-C motif chemokine 28 isoform a
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 28
• NCBI Official Symbol: CCL28
• NCBI Official Synonym Symbols: MEC; CCK1; SCYA28
• NCBI Protein Information: C-C motif chemokine 28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL28
• UniProt Synonym Gene Names: SCYA28; MEC
• UniProt Entry Name: CCL28_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: extracellular region

Molecular Function: chemokine activity; protein binding

Biological Process: chemotaxis; positive regulation of cell-matrix adhesion