CCL28 recombinant protein

CCL28, 23-127aa, Human, His tag, E Coli

Cat. Num. AAA203388

• Gene Names: CCL28; MEC; CCK1; SCYA28
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CCL28; 23-127aa; Human; His tag; E Coli; Chemokine (C-C motif) ligand 28; CCK1; MEC; SCYA28; CC chemokine CCL28; mucosae-associated epithelial chemokine; small inducible cytokine subfamily A (Cys-Cys) member 28; small inducible cytokine A28; ; CCL28 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 10mM Sodium citrate pH3.5 containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MILPIASSCC TEVSHHISRR LLERVNMCRI QRADGDCDLA AVILHVKRRR ICVSPHNHTV KQWMKVQAAK KNGKGNVCHR KKHHGKRNSN RAHQGKHETY GHKTPY
• Sequence Length: 127

• Applicable Applications for CCL28 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CCL28 recombinant protein

CCL28, also known as chemokine (C-C motif) ligand 28 or mucosae-associated epithelial chemokine (MEC), belongs to the subfamily of small cytokine CC proteins. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. Recombinant CCL28 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CCL28 recombinant protein

Hormones & Cytokines

References

Pacheco-Rodriguez G., et al. (2009) J Immunol. 182 (3):1270-7. ; Sundstrom P., et al. (2008) Eur J Immunol. 38 (12):3327-38.;

NCBI and Uniprot Product Information

• NCBI GI #: 22538811
• NCBI GeneID: 56477
• NCBI Accession #: NP_683513
• NCBI GenBank Nucleotide #: NM_148672.3
• UniProt Accession #: Q9NRJ3; D7RIE7
• Molecular Weight: 14.3 kDa (126aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: C-C motif chemokine 28 isoform a
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 28
• NCBI Official Symbol: CCL28
• NCBI Official Synonym Symbols: MEC; CCK1; SCYA28
• NCBI Protein Information: C-C motif chemokine 28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL28
• UniProt Synonym Gene Names: SCYA28; MEC

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.