Mouse C-C motif chemokine 28 (CCL28) ELISA Kit | CCL28 elisa kit

Mouse C-C motif chemokine 28, CCL28 ELISA Kit

Cat. Num. AAA911564

• Gene Names: Ccl28; MEC; CCK1; Scya28
• Reactivity: Mouse
• Synonyms: C-C motif chemokine 28 (CCL28); Mouse C-C motif chemokine 28; CCL28 ELISA Kit; Mouse C-C motif chemokine 28 (CCL28) ELISA kit; CCK1; MEC; MGC71902; SCYA28; CC chemokine CCL28; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys); member 28; CCL28 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CCL2. No significant cross-reactivity or interference between human CCL2 and analogues was observed.
• Sequence Length: 130

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL28 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 9931984
• NCBI GeneID: 56838
• NCBI Accession #: NP_064675.1
• NCBI GenBank Nucleotide #: NM_020279.3
• UniProt Accession #: Q9JIL2
• Molecular Weight: 14,570 Da
• NCBI Official Full Name: C-C motif chemokine 28
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 28
• NCBI Official Symbol: Ccl28
• NCBI Official Synonym Symbols: MEC; CCK1; Scya28
• NCBI Protein Information: C-C motif chemokine 28; small inducible cytokine A28; small-inducible cytokine A28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl28
• UniProt Synonym Gene Names: Scya28
• UniProt Entry Name: CCL28_MOUSE

Uniprot Description

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: chemokine activity; cytokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion; chemotaxis