Human CCL28 PicoKine ELISA Kit | CCL28 elisa kit

Human CCL28 PicoKine ELISA Kit

Cat. Num. AAA177576

• Gene Names: CCL28; MEC; CCK1; SCYA28
• Reactivity: Human
• Synonyms: CCL28 PicoKine; Human CCL28 PicoKine ELISA Kit; C-C motif chemokine 28; CCK 1; CCK1; CCL 28; Chemokine (C-C motif) ligand 28; MEC; MGC71902; Mucosae associated epithelial chemokine; OTTHUMP0000022162; SCYA28; Small inducible cytokine A28; Small inducible cytokine A28 [Precursor]; Small inducible cytokine subfamily A (Cys-Cys); member 28; chemokine (C-C motif) ligand 28; CCL28 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL28
• Sequence Length: 127

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human CCL28 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, I23-Y127
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human CCL28 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL28 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|223|820|1587|426|975|1628; Standard deviation|11.60|53.3|109.50|27.69|70.2|135.12; CV(%)|5.2|6.5|6.9|6.5|7.2|8.3
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.019|0.137|0.275|0.476|0.857|1.532|2.039|2.324
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human CCL28 PicoKine ELISA Kit standard curve )

Related Product Information for CCL28 elisa kit

Principle of the assay: Human CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL28 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: I23-Y127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL28 amount of sample captured in plate.

Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

NCBI and Uniprot Product Information

• NCBI GI #: 687569447
• NCBI GeneID: 56477
• NCBI Accession #: NP_001288802.1
• NCBI GenBank Nucleotide #: NM_001301873.1
• UniProt Accession #: Q9NRJ3; D7RIE7
• Molecular Weight: 12,375 Da
• NCBI Official Full Name: C-C motif chemokine 28 isoform a
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 28
• NCBI Official Symbol: CCL28
• NCBI Official Synonym Symbols: MEC; CCK1; SCYA28
• NCBI Protein Information: C-C motif chemokine 28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL28
• UniProt Synonym Gene Names: SCYA28; MEC
• UniProt Entry Name: CCL28_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: chemokine activity; protein binding

Biological Process: chemotaxis; elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion