Typical Testing Data/Standard Curve (for reference only)
Human Rantes ELISA Kit | CCL5 elisa kit
Rantes ELISA
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RANK polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RANK polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RANK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RANK can be calculated!!Background/Introduction: Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein. It is expressed on the surface of osteoclasts, dendritic cells and facilitates immune signaling. RANK is involved in their activation upon ligand binding. RANKL (Receptor Activator for Nuclear Factor kappa B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. RANKL and RANK have an essential role in the brain. RANK and RANKL appear to be important regulators of interactions between T cells and dendritic cells.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: extracellular space; cytoplasm; extracellular region
Molecular Function: protein homodimerization activity; protein self-association; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; protein binding; CCR1 chemokine receptor binding; CCR4 chemokine receptor binding; chemokine activity; chemokine receptor binding; chemokine receptor antagonist activity; CCR5 chemokine receptor binding; phospholipase activator activity; chemoattractant activity
Biological Process: regulation of chronic inflammatory response; positive regulation of cell adhesion; exocytosis; response to toxin; positive regulation of smooth muscle cell proliferation; positive regulation of JAK-STAT cascade; chemotaxis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of homotypic cell-cell adhesion; positive regulation of translational initiation; monocyte chemotaxis; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; positive regulation of innate immune response; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; dendritic cell chemotaxis; positive regulation of T cell proliferation; inflammatory response; protein tetramerization; phospholipase D activation; positive regulation of viral genome replication; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; response to virus; MAPKKK cascade; positive regulation of cellular biosynthetic process; macrophage chemotaxis; positive regulation of phosphoinositide 3-kinase cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; cellular protein complex assembly; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of viral genome replication; eosinophil chemotaxis; positive regulation of calcium ion transport; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; regulation of T cell activation
Research Articles on CCL5
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Product Notes
The Human CCL5 ccl5 (Catalog #AAA494816) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494816 ELISA Kit recognizes Human CCL5. It is sometimes possible for the material contained within the vial of "Rantes, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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