Testing Data
Calpain 1 Active Protein | CAPN1 active protein
Active Porcine Calpain 1
Applications
SDS-Page
Purity
>=85%
Synonyms
Calpain 1; Active Porcine Calpain 1; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; CAPN1 active protein
Host
Porcine Erythrocytes
Purity/Purification
>=85%
Form/Format
In 20 mM imidazole-HCl, 5 mM beta-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
Appearance: Liquid
Appearance: Liquid
Sequence Length
714
Applicable Applications for CAPN1 active protein
SDS-PAGE; HPLC
Biological Activity
>= 1000 units/mg protein prior to freezing.
Results
>= 1000 units/mg
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25 degree C using the Calpain Activity Assay Kit, (K240-100).
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -70 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for CAPN1 active protein
Background: The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
Product Categories/Family for CAPN1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110 kDa
NCBI Official Full Name
calpain-1 catalytic subunit isoform X1
NCBI Official Symbol
CAPN1
NCBI Protein Information
calpain-1 catalytic subunit
UniProt Protein Name
Calpain-1 catalytic subunit
Protein Family
UniProt Gene Name
CAPN1
UniProt Synonym Gene Names
CANP 1; muCANP
UniProt Entry Name
CAN1_PIG
Uniprot Description
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Research Articles on CAPN1
Similar Products
Product Notes
The CAPN1 capn1 (Catalog #AAA844023) is an Active Protein produced from Porcine Erythrocytes and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Calpain 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC. Researchers should empirically determine the suitability of the CAPN1 capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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