Typical Testing Data/Standard Curve (for reference only)
Human MCP-2 ELISA Kit | MCP-2 elisa kit
MCP-2 ELISA
Gene Names
Mcpt2; Mcp-2; MMCP-2
Reactivity
Human
Synonyms
MCP-2; MCP-2 ELISA; MCP-2 elisa kit
Reactivity
Human
Sequence Length
244
Samples
Mouse Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<5pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MCP-2 elisa kit
Intended Uses: For detection of L-Selectin in Mouse serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-L-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-L-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-Selectin amount of sample captu red in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-Selectin can be calculated!!Background/Introduction: L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. It may also play a role in neutrophil adhesion to endothelium at sites of inflammation.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-L-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-L-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-Selectin amount of sample captu red in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-Selectin can be calculated!!Background/Introduction: L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. It may also play a role in neutrophil adhesion to endothelium at sites of inflammation.
Product Categories/Family for MCP-2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,732 Da
NCBI Official Full Name
mast cell protease 2
NCBI Official Synonym Full Names
mast cell protease 2
NCBI Official Symbol
Mcpt2
NCBI Official Synonym Symbols
Mcp-2; MMCP-2
NCBI Protein Information
mast cell protease 2
UniProt Protein Name
Mast cell protease 2
UniProt Gene Name
Mcpt2
UniProt Synonym Gene Names
mMCP-2
UniProt Entry Name
MCPT2_MOUSE
Uniprot Description
Mcpt2: Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: EC 3.4.21.-; Protease
Cellular Component: extracellular space; intracellular
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: protein processing; proteolysis
Research Articles on MCP-2
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Product Notes
The Human MCP-2 mcpt2 (Catalog #AAA494796) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494796 ELISA Kit recognizes Human MCP-2. It is sometimes possible for the material contained within the vial of "MCP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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