Mouse Connective Tissue Growth Factor (CTGF) CLIA Kit | CTGF clia kit

Mouse Connective Tissue Growth Factor (CTGF) CLIA Kit

Cat. Num. AAA2708447

• Gene Names: Ccn2; Ctgf; Hcs24; Fisp12; fisp-12
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Connective Tissue Growth Factor (CTGF); Mouse Connective Tissue Growth Factor (CTGF) CLIA Kit; Connective Tissue Growth Factor; CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2; CTGF clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF).; No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.
• Sequence Length: 348

• Applicable Applications for CTGF clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 171846283
• NCBI GeneID: 14219
• NCBI Accession #: NP_034347.2
• NCBI GenBank Nucleotide #: NP_034347.2
• UniProt Accession #: P29268
• NCBI Official Full Name: CCN family member 2
• NCBI Official Synonym Full Names: cellular communication network factor 2
• NCBI Official Symbol: Ccn2
• NCBI Official Synonym Symbols: Ctgf; Hcs24; Fisp12; fisp-12
• NCBI Protein Information: CCN family member 2
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: Ctgf
• UniProt Synonym Gene Names: Ccn2; Fisp-12; Fisp12; Hcs24

Uniprot Description

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (). Enhances fibroblast growth factor-induced DNA synthesis ().