Typical Testing Data
Human Interleukin enhancer-binding factor 3 ELISA Kit | ILF3 elisa kit
Human Interleukin enhancer-binding factor 3 ELISA Kit
Reactivity
Human
Synonyms
Interleukin enhancer-binding factor 3; Human Interleukin enhancer-binding factor 3 ELISA Kit; ILF3/Translational control protein 80 (TCP80)/Nuclear factor of activated T-cells 90 kDa/NF-AT-90/Nuclear factor associated with dsRNA (NFAR)/Double-stranded RNA-binding protein 76 (DRBP76)/M-phase phosphoprotein 4 (MPP4)/DRBF/MPHOSPH4/NF90; ILF3 elisa kit
Reactivity
Human
Sequence Length
323
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
15.6-1000pg/ml
Sensitivity
9.375pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data
Typical Testing Data
Typical Standard Curve
Typical Standard Curve
Related Product Information for ILF3 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ILF3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ILF3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ILF3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ILF3 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
34,325 Da
NCBI Official Full Name
interleukin enhancer binding factor 3
UniProt Protein Name
Forkhead box protein K2
Protein Family
UniProt Gene Name
FOXK2
UniProt Synonym Gene Names
ILF; ILF1
UniProt Entry Name
FOXK2_HUMAN
Uniprot Description
FOXK2: Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus
Molecular Function: magnesium ion binding; protein binding; sequence-specific DNA binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter
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Product Notes
The Human ILF3 foxk2 (Catalog #AAA763051) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA763051 ELISA Kit recognizes Human ILF3. It is sometimes possible for the material contained within the vial of "Interleukin enhancer-binding factor 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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