Connective Tissue Growth Factor (CTGF) Recombinant Protein | CTGF recombinant protein

Recombinant Connective Tissue Growth Factor (CTGF)

Cat. Num. AAA2010435

• Gene Names: Ctgf; Ccn2; Hcs24; Fisp12; fisp-12
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Connective Tissue Growth Factor (CTGF); Recombinant Connective Tissue Growth Factor (CTGF); CTGF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-LEENIKK GKKCIRTPKI AKPVKFELSG CTSVKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEI MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGD
• Sequence Length: 348

• Applicable Applications for CTGF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu245~Asp346 (Accession # P29268) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 171846283
• NCBI GeneID: 14219
• NCBI Accession #: NP_034347.2
• NCBI GenBank Nucleotide #: NM_010217.2
• UniProt Accession #: P29268; Q922U0; G5E830
• Molecular Weight: 13.2kDa
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: Ctgf
• NCBI Official Synonym Symbols: Ccn2; Hcs24; Fisp12; fisp-12
• NCBI Protein Information: connective tissue growth factor; CCN family member 2; fibroblast inducible secreted protein; fibroblast inducible secreated protein; hypertrophic chondrocyte-specific protein 24; hypertrophic chondrocyte-specific gene product 24; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24)
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: Ctgf
• UniProt Synonym Gene Names: Ccn2; Fisp-12; Fisp12; Hcs24
• UniProt Entry Name: CTGF_MOUSE

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide

Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity; fibronectin binding

Biological Process: integrin-mediated signaling pathway; ossification; cell migration; fibroblast growth factor receptor signaling pathway; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of caspase activity; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; tissue homeostasis; regulation of chondrocyte differentiation; angiogenesis; positive regulation of protein amino acid phosphorylation; regulation of cell growth; cell differentiation; cell adhesion; positive regulation of cell differentiation; positive regulation of cell activation; cartilage condensation; lung development