Western Blot
(Western blot analysis of Hela cell lysate(lane1), Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with beta-Actin mouse mAb(6G3) diluted at 1:5000.)
beta-Actin Monoclonal Antibody | anti-ACTB antibody
beta-Actin Mouse Monoclonal (6G3)Antibody
Reactivity
Human, Rat, Mouse, Rabbit, Sheep, Pig, Chicken
Applications
Western Blot, Immunohistochemistry
Synonyms
beta-Actin; Monoclonal Antibody; beta-Actin Mouse Monoclonal (6G3)Antibody; anti-ACTB antibody
Reactivity
Human, Rat, Mouse, Rabbit, Sheep, Pig, Chicken
Clonality
Monoclonal
Isotype
IgG1
Clone Number
R6G3
Form/Format
Liquid
Sequence Length
159
Applicable Applications for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:5000
IHC: 1:400
IHC: 1:400
Immunogen
Full-length beta-Actin protein of human.
Preparation and Storage
Stable for 1 year at-20 degree C. For long term storage, Aliquot and store at-20 degree C or below. Avoid repeated freezing and thawing cycles
Western Blot
(Western blot analysis of Hela cell lysate(lane1), Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with beta-Actin mouse mAb(6G3) diluted at 1:5000.)
Western Blot
(Western blot analysis of Hela cell lysate(lane1), Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with beta-Actin mouse mAb(6G3) diluted at 1:5000.)
Immunohistochemistry
(Immunohistochemistry Staining of Human ovary tissue with beta -Actin mouse mAb(6G3) diluted at 1:200..)
Immunohistochemistry
(Immunohistochemistry Staining of Human ovary tissue with beta -Actin mouse mAb(6G3) diluted at 1:200..)
Related Product Information for anti-ACTB antibody
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore beta-Actin should not be used as loading control for these tissues.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,739 Da
NCBI Official Full Name
beta-actin, partial
UniProt Protein Name
Beta-actin
Protein Family
Similar Products
Product Notes
The ACTB (Catalog #AAA850254) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The beta-Actin Mouse Monoclonal (6G3)Antibody reacts with Human, Rat, Mouse, Rabbit, Sheep, Pig, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:5000 IHC: 1:400. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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