Rabbit beta-Actin Polyclonal Antibody | anti-ACTB antibody

beta-Actin Antibody (biotin)

Cat. Num. AAA154470

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Drosophila
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: beta-Actin; Polyclonal Antibody; beta-Actin Antibody (biotin); Biotin-Actin; Beta-Actin; Actin; actin; beta; anti-ACTB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Drosophila
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Biotin-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-ACTB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western blot at 0.5 - 1 mu/ml.
• Conjugate: Biotin
• Immunogen: Biotin-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.
• Buffer: Biotin-Beta-Actin antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of Actin in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, THP-1, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken small intestine, and drosophila lysate with Biotin-Beta-Actin antibody at 1 μg/mL.)

Related Product Information for anti-ACTB antibody

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Product Categories/Family for anti-ACTB antibody

Loading Control

References

1. Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909. 2. Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.

NCBI and Uniprot Product Information

• NCBI GI #: 12803203
• NCBI GeneID: 60
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: Predicted: 42 kDa; Observed: 42 kDa
• NCBI Official Full Name: Actin, beta
• NCBI Official Synonym Full Names: actin beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Beta-actin-like protein
• UniProt Gene Name: ACTB

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; membrane; NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; plasma membrane; protein complex; ribonucleoprotein complex; vesicle

Molecular Function: identical protein binding; kinesin binding; nitric-oxide synthase binding; nucleosomal DNA binding; protein binding; structural constituent of cytoskeleton; Tat protein binding

Biological Process: ATP-dependent chromatin remodeling; cell motility; ephrin receptor signaling pathway; positive regulation of gene expression, epigenetic; protein deubiquitination; retinal homeostasis; substantia nigra development; vascular endothelial growth factor receptor signaling pathway

Disease: Baraitser-winter Syndrome 1; Dystonia, Juvenile-onset