Western Blot (WB)
Rabbit Actin-beta Polyclonal Antibody | anti-ACTB antibody
Rabbit anti Actin-beta Polyclonal Antibody
Reactivity
Human,Mouse,Rat,Dog,Bovine,Sheep,Goat
Applications
Immunohistochemistry, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by EpitopeAffinity Purification.
Synonyms
Actin-beta; Polyclonal Antibody; Rabbit anti Actin-beta Polyclonal Antibody; anti-ACTB antibody
Host
Rabbit
Reactivity
Human,Mouse,Rat,Dog,Bovine,Sheep,Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes actinprotein.It cross-reacts to human, mouse, rat, chicken, bovine etc.
Purity/Purification
The Rabbit IgG is purified by EpitopeAffinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
Sequence Length
375
Applicable Applications for anti-ACTB antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot 0.1-1
ug/m ELISA 0.01-0.1 ug/m
Immunoprecipitation 2-5 ug/m
IHC 2-5 ug/ml
Flow cytometry 10-20 ug per 106 cells
Optimal dilutions should be determined by researchers for the specific applications.
ug/m ELISA 0.01-0.1 ug/m
Immunoprecipitation 2-5 ug/m
IHC 2-5 ug/ml
Flow cytometry 10-20 ug per 106 cells
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A synthetic peptide corresponding to N-term of human beta-Actin protein. This sequence is identical in human, mouse, rat, bovine and chicken
Positive Control
Leiomyoma and skin
Cellular Location
Cytoplasmic/Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-ACTB antibody
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three Alpha-actin (skeletal, cardiac, and smooth muscle), one (Beta-non-muscle), and two Gamma-actins (Gamma-smooth muscle and Gamma-non-muscle). Antibody to Beta-actin provides a specific and useful tool in studying the intracellular distribution of Beta-actin and the static and dynamic aspects of the cytoskeleton. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This beta-actin is a major constituent of the contractile apparatus and one of the two non-muscle cytoskeletal actins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~42 kDa
NCBI Official Full Name
actin beta
NCBI Official Symbol
ACTB
NCBI Protein Information
actin beta; actin, cytoplasmic 1
Protein Family
Similar Products
Product Notes
The ACTB (Catalog #AAA460213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Actin-beta Polyclonal Antibody reacts with Human,Mouse,Rat,Dog,Bovine,Sheep,Goat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin-beta can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunohistochemistry (IHC). Western Blot 0.1-1 ug/m ELISA 0.01-0.1 ug/m Immunoprecipitation 2-5 ug/m IHC 2-5 ug/ml Flow cytometry 10-20 ug per 106 cells Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
