Western Blot (WB)
Rabbit Beta-Actin Polyclonal Antibody
Anti-Beta-Actin antibody
Reactivity
Human, mouse, rat
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
Beta-Actin; Polyclonal Antibody; Anti-Beta-Actin antibody; Polyclonal Anti- Beta-Actin; anti-Beta-Actin antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-Beta-Actin antibody
Western Blot (WB)
Application Notes
WB (1:1000-2000)
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the region of Beta-Actin.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-Beta-Actin antibody
Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.
References
1. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 2. Ji, Y., Ferracci, G., Warley, A., Ward, M., Leung, K.-Y., Samsuddin, S., Leveque, C., Queen, L., Reebye, V., Pal, P., Gkaliagkousi, E., Seager, M., Ferro, A. Beta-actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc. Nat. Acad. Sci. 104: 8839-8844, 2007
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
beta-actin, partial
Protein Family
Similar Products
Product Notes
The Beta-Actin (Catalog #AAA355174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Beta-Actin antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB (1:1000-2000). Researchers should empirically determine the suitability of the Beta-Actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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