Western Blot (WB)
(Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.)
Rabbit beta-Actin Polyclonal Antibody | anti-ACTB antibody
Anti-beta-Actin Rabbit Polyclonal Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Synonyms
beta-Actin; Polyclonal Antibody; Anti-beta-Actin Rabbit Polyclonal Antibody; ACTB; Actin; cytoplasmic 1; Beta-actin; anti-ACTB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
The antibody detects endogenous beta-actin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:3000
IHC-P: 1:200
IHC-P: 1:200
Tag
Actin cytoplasmic 1
Observed Band (KD)
43
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.)
Western Blot (WB)
(Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.)
Immunohistochemistry (IHC)
(Fig.2. IHC-P staining of Human ovary tissue, diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.2. IHC-P staining of Human ovary tissue, diluted at 1:200.)
Related Product Information for anti-ACTB antibody
Description: Rabbit Anti-beta-actin Polyclonal Antibody
Background: ACTB encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Background: ACTB encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Product Categories/Family for anti-ACTB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
375
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
Protein Family
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
Similar Products
Product Notes
The ACTB actb (Catalog #AAA9719104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Actin Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:3000 IHC-P: 1:200. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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