SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Alpha 1 Antichymotrypsin Recombinant Protein | SERPINA3 recombinant protein
Alpha 1 Antichymotrypsin protein (His tag)
Gene Names
SERPINA3; ACT; AACT; GIG24; GIG25
Applications
SDS-Page
Purity
> 95% pure
Synonyms
Alpha 1 Antichymotrypsin; Alpha 1 Antichymotrypsin protein (His tag); Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3.; member 3 Cell growth inhibiting gene 24/25 protein protein; Antichymotrypsin protein; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase protein; antitrypsin) member 3 protein; MGC88254 protein; SERPINA3 protein; GIG24 protein; Growth inhibiting protein 24 protein; GIG25 protein; clade A protein; Serpin peptidase inhibitor protein; alpha-1-Antichymotrypsin; Serine (or cysteine) proteinase inhibitor clade A member 3 protein; Growth inhibiting protein 25 protein; Serine proteinase inhibitor clade A member 3 protein; AACT protein; alpha-1-Antichymotrypsin protein; ACT protein; SERPINA3 recombinant protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, and 1mM DTT.
Concentration
1 mg/ml (varies by lot)
Sequence
24-423 amino acids: MGSSHHHHHH SSGLVPRGSH MHPNSPLDEE NLTQENQDRG THVDLGLASA NVDFAFSLYK QLVLKAPDKN VIFSPLSIST ALAFLSLGAH NTTLTEILKG LKFNLTETSE AEIHQSFQHL LRTLNQSSDE LQLSMGNAMF VKEQLSLLDR FTEDAKRLYG SEAFATDFQD SAAAKKLIND YVKNGTRGKI TDLIKDLDSQ TMMVLVNYIF FKAKWEMPFD PQDTHQSRFY LSKKKWVMVP MMSLHHLTIP YFRDEELSCT VVELKYTGNA SALFILPDQD KMEEVEAMLL PETLKRWRDS LEFREIGELY LPKFSISRDY NLNDILLQLG IEEAFTSKAD LSGITGARNL AVSQVVHKAV LDVFEEGTEA SAATAVKITL LSALVETRTI VRFNRPFLMI IVPTDTQNIF FMSKVTNPKQ A
Sequence Length
423
Applicable Applications for SERPINA3 recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
Alpha-1-antichymotrypsin is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. Recombinant alpha-1-antichymotrypsin protein was expressed in E Coli and purified by using conventional chromatography techniques.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for SERPINA3 recombinant protein
Purified recombinant Human Alpha 1 Antichymotrypsin protein
Product Categories/Family for SERPINA3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.6 kDa (421aa), confirmed by MALDI-TOF.
NCBI Official Full Name
alpha-1-antichymotrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
NCBI Official Symbol
SERPINA3
NCBI Official Synonym Symbols
ACT; AACT; GIG24; GIG25
NCBI Protein Information
alpha-1-antichymotrypsin
UniProt Protein Name
Alpha-1-antichymotrypsin
Protein Family
UniProt Gene Name
SERPINA3
UniProt Synonym Gene Names
AACT; ACT
UniProt Entry Name
AACT_HUMAN
NCBI Description
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]
Uniprot Description
SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 14q32.1
Cellular Component: extracellular space; extracellular region; intracellular; nucleus
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; DNA binding
Biological Process: regulation of lipid metabolic process; maintenance of gastrointestinal epithelium; acute-phase response; inflammatory response
Research Articles on SERPINA3
Similar Products
Product Notes
The SERPINA3 serpina3 (Catalog #AAA836684) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha 1 Antichymotrypsin can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SERPINA3 serpina3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 24-423 amino acids: MGSSHHHHHH SSGLVPRGSH MHPNSPLDEE NLTQENQDRG THVDLGLASA NVDFAFSLYK QLVLKAPDKN VIFSPLSIST ALAFLSLGAH NTTLTEILKG LKFNLTETSE AEIHQSFQHL LRTLNQSSDE LQLSMGNAMF VKEQLSLLDR FTEDAKRLYG SEAFATDFQD SAAAKKLIND YVKNGTRGKI TDLIKDLDSQ TMMVLVNYIF FKAKWEMPFD PQDTHQSRFY LSKKKWVMVP MMSLHHLTIP YFRDEELSCT VVELKYTGNA SALFILPDQD KMEEVEAMLL PETLKRWRDS LEFREIGELY LPKFSISRDY NLNDILLQLG IEEAFTSKAD LSGITGARNL AVSQVVHKAV LDVFEEGTEA SAATAVKITL LSALVETRTI VRFNRPFLMI IVPTDTQNIF FMSKVTNPKQ A. It is sometimes possible for the material contained within the vial of "Alpha 1 Antichymotrypsin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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