Glutaredoxin3 Recombinant Protein | GLRX3 recombinant protein

Glutaredoxin3 protein (His tag)

Cat. Num. AAA836807

• Gene Names: GLRX3; GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3
• Applications: SDS-Page
• Purity: > 95% pure
• Synonyms: Glutaredoxin3; Glutaredoxin3 protein (His tag); thioredoxin-like 2 protein; Glutaredoxin 3; Glutaredoxin-3; PICOT protein; GLRX3 protein; glutaredoxin 3 protein; Glutaredoxin 3 protein; Thioredoxin-like protein 2 protein; Glutaredoxin-3 protein; GLRX4 protein; GRX4 protein; glutaredoxin 4 protein; bA500G10.4 protein; TXNL2 protein; GRX3 protein; GLRX3 recombinant protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: 1-335 amino acids: MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN
• Sequence Length: 335

• Applicable Applications for GLRX3 recombinant protein: SDS-PAGE
• Protein Type: Recombinant
• Biological Significance: Glutaredoxin(GRX), also known as thioltransferase, is a member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
• Expression System: E Coli
• Tag/Conjugate: His tag
• Preparation and Storage: Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Figure annotation denotes ug of protein loaded and % gel used.)

Related Product Information for GLRX3 recombinant protein

Purified recombinant Human Glutaredoxin3 protein

Product Categories/Family for GLRX3 recombinant protein

Proteases; Inhibitors; & Enzymes; Conjugated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 315467838
• NCBI GeneID: 10539
• NCBI Accession #: NP_001186797.1
• NCBI GenBank Nucleotide #: NM_001199868.1
• UniProt Accession #: O76003; Q5JV01; Q96CE0; Q9P1B0; Q9P1B1; B3KMP7; B3KMQ5; D3DRG2
• Molecular Weight: 39.6 kDa (355aa), confirmed by MALDI-TOF
• NCBI Official Full Name: glutaredoxin-3
• NCBI Official Synonym Full Names: glutaredoxin 3
• NCBI Official Symbol: GLRX3
• NCBI Official Synonym Symbols: GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3
• NCBI Protein Information: glutaredoxin-3
• UniProt Protein Name: Glutaredoxin-3
• Protein Family: Glutaredoxin
• UniProt Gene Name: GLRX3
• UniProt Synonym Gene Names: PICOT; TXNL2; PICOT; PKCq-interacting protein
• UniProt Entry Name: GLRX3_HUMAN

NCBI Description

This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

GLRX3: Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: cell cortex; Z disc

Molecular Function: protein binding; protein kinase C binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; iron-sulfur cluster binding

Biological Process: cell redox homeostasis; regulation of the force of heart contraction