Western Blot (WB)
(Western Blot; Sample: Recombinant WNT5A, Mouse.)
Mouse anti-Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Monoclonal Antibody | anti-WNT5A antibody
Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant WNT5A, Mouse.)
Western Blot (WB)
(Western Blot; Sample: Recombinant WNT5A, Mouse.)
Western Blot (WB)
(Western Blot;Sample: Rat Liver lysatePrimary Ab: 2ug/ml Mouse Anti-Mouse WNT5A AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035 ))
Western Blot (WB)
(Western Blot;Sample: Rat Liver lysatePrimary Ab: 2ug/ml Mouse Anti-Mouse WNT5A AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035 ))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Cerebrum Tissue;Primary Ab: 30ug/ml Mouse Anti-Mouse WNT5A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035 ))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Cerebrum Tissue;Primary Ab: 30ug/ml Mouse Anti-Mouse WNT5A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035 ))
NCBI and Uniprot Product Information
Uniprot Description
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Research Articles on WNT5A
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Product Notes
The WNT5A wnt5a (Catalog #AAA2111713) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the WNT5A wnt5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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