Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) RTU ELISA Kit | WNT5A rtu elisa kit

Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit

Cat. Num. AAA4503395

• Gene Names: Wnt5a; Wnt-5a; 8030457G12Rik
• Reactivity: Mouse
• Synonyms: Wingless Type MMTV Integration Site Family; Member 5A (WNT5A); Mouse Wingless Type MMTV Integration Site Family; Member 5A (WNT5A) ELISA Kit; WNT5A rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 360

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 12.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT5A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 371940979
• NCBI GeneID: 22418
• NCBI Accession #: NP_001243153.1
• NCBI GenBank Nucleotide #: NM_001256224.2
• UniProt Accession #: P22725; Q8BM17; Q8BMF9; Q8VCV6
• Molecular Weight: 40,489 Da
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 5A
• NCBI Official Symbol: Wnt5a
• NCBI Official Synonym Symbols: Wnt-5a; 8030457G12Rik
• NCBI Protein Information: protein Wnt-5a
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: Wnt5a
• UniProt Synonym Gene Names: Wnt-5a

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors (PubMed:17117926). Can activate or inhibit canonical Wnt signaling, depending on receptor context (PubMed:16602827). In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (PubMed:16602827). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration (PubMed:17117926). Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells (). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle (PubMed:10021340). Inhibits type II collagen expression in chondrocytes ().