Typical Testing Data/Standard Curve (for reference only)
Mouse Protein Wnt-5a ELISA Kit | Wnt5a elisa kit
Mouse Protein Wnt-5a ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: cytokine activity; frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor binding; receptor tyrosine kinase-like orphan receptor binding; transcription factor activity
Biological Process: activation of JNK activity; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of protein kinase B; ameboidal cell migration; anterior/posterior pattern formation; axon guidance; axonal fasciculation; cartilage development; cell differentiation; cell fate commitment; cell migration; cell-cell signaling; cervix development; convergent extension; convergent extension involved in axis elongation; convergent extension involved in organogenesis; determination of anterior/posterior axis, embryo; determination of left/right symmetry; development of primary male sexual characteristics; digestive tract morphogenesis; embryonic development; embryonic digit morphogenesis; embryonic limb morphogenesis; embryonic skeletal development; establishment of epithelial cell polarity; establishment of planar polarity; genitalia development; heart looping; hindgut morphogenesis; hypophysis morphogenesis; inner ear morphogenesis; JNK cascade; limb morphogenesis; lung development; male gonad development; midbrain development; midgut development; morphogenesis of an epithelium; multicellular organismal development; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of melanin biosynthetic process; negative regulation of synaptogenesis; negative regulation of transcription, DNA-dependent; neural tube closure; neural tube development; neurite morphogenesis; neurogenesis; notochord morphogenesis; olfactory bulb interneuron development; organ morphogenesis; palate development; paraxial mesoderm formation; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of cGMP metabolic process; positive regulation of chemokine biosynthetic process; positive regulation of cytokine production; positive regulation of cytokine secretion during immune response; positive regulation of endocytosis; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of macrophage activation; positive regulation of meiosis; positive regulation of mesenchymal cell proliferation; positive regulation of ossification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of protein localization; signal transduction; somitogenesis; urinary bladder development; uterus development; vagina development; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; wound healing
Research Articles on Wnt5a
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Product Notes
The Mouse Wnt5a wnt5a (Catalog #AAA2885295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885295 ELISA Kit recognizes Mouse Wnt5a. It is sometimes possible for the material contained within the vial of "Protein Wnt-5a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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