Activity Data
(Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of WNT5A and WIF1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Active Protein | WNT5A active protein
Active Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Activity Data
(Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of WNT5A and WIF1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Besides, WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT5A and recombinant rat WIF1. Briefly, WNT5A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to WIF1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-WNT5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of WNT5A and WIF1 was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS-Page
SDS-Page
Testing Data
Testing Data
Testing Data
Testing Data
NCBI and Uniprot Product Information
Accurate Molecular Weight: 62kDa
Uniprot Description
WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14 A3|14 16.8 cM
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: cytokine activity; DNA binding transcription factor activity; frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor binding; receptor tyrosine kinase-like orphan receptor binding
Biological Process: activation of JNK activity; activation of MAPK activity; activation of protein kinase B activity; ameboidal cell migration; anterior/posterior axis specification, embryo; anterior/posterior pattern specification; axon guidance; axonal fasciculation; axonogenesis; cartilage development; cell differentiation; cell fate commitment; cell migration; cell-cell signaling; cervix development; convergent extension; convergent extension involved in axis elongation; convergent extension involved in organogenesis; determination of left/right symmetry; development of primary male sexual characteristics; digestive tract morphogenesis; embryonic development; embryonic digit morphogenesis; embryonic limb morphogenesis; embryonic skeletal system development; establishment of epithelial cell apical/basal polarity; establishment of planar polarity; genitalia development; heart looping; hindgut morphogenesis; hypophysis morphogenesis; inner ear morphogenesis; JNK cascade; limb morphogenesis; lung development; male gonad development; midbrain development; midgut development; morphogenesis of an epithelium; multicellular organism development; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of melanin biosynthetic process; negative regulation of synaptogenesis; negative regulation of transcription, DNA-dependent; neural tube closure; neural tube development; neurogenesis; neuron projection morphogenesis; notochord morphogenesis; olfactory bulb interneuron development; organ morphogenesis; palate development; paraxial mesoderm formation; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of cGMP metabolic process; positive regulation of chemokine biosynthetic process; positive regulation of cytokine production; positive regulation of cytokine secretion involved in immune response; positive regulation of endocytosis; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of macrophage activation; positive regulation of meiosis; positive regulation of mesenchymal cell proliferation; positive regulation of NF-kappaB transcription factor activity; positive regulation of ossification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein kinase activity; positive regulation of protein phosphorylation; positive regulation of timing of anagen; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; regulation of protein localization; signal transduction; somitogenesis; urinary bladder development; uterus development; vagina development; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; Wnt signaling pathway; wound healing
Research Articles on WNT5A
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Product Notes
The WNT5A wnt5a (Catalog #AAA2097323) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the WNT5A wnt5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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