Mouse anti-Human CD24 Monoclonal Antibody | anti-CD24 antibody

Anti-Hu CD24 Pacific Blue

• Gene Names: CD24; CD24A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD24; Monoclonal Antibody; Anti-Hu CD24 Pacific Blue; HSA; nectadorin; SCLC4; CD24 molecule; anti-CD24 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: SN3
• Specificity: The antibody SN3 reacts with CD24, a 35-45kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD24 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Glycoproteins purified from human NALM-1 cell line.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD24 (SN3) Pacific Blue™.)

Related Product Information for anti-CD24 antibody

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

References

*Všianská P, ?íhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543,*Maliar A, Servais C, Waks T, Chmielewski M, Lavy R, Altevogt P, Abken H, Eshhar Z: Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology. 2012 Nov;143(5):1375-1384, URL: http://www.ncbi.nlm.nih.gov/pubmedterm=maliar%20a,*Fukukawa T, Matsuzaki H, Haruta Y, Hara H, Seon BK: New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells. Exp Hematol. 1986 Oct;14(9):850-5., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Barcos M, Pollard C, Fukukawa T, Seon BK: Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3. Hematol Oncol. 1986 Oct-Dec;4(4):251-9., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992 Feb 1;175(2):397-404., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990 Jan 15;144(2):638-41., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353
*Runz S, Mierke CT, Joumaa S, Behrens J, Fabry B, Altevogt P: CD24 induces localization of beta1 integrin to lipid raft domains. Biochem Biophys Res Commun. 2008 Jan 4;365(1):35-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/17980703ordin,*Chou YY, Jeng YM, Lee TT, Hu FC, Kao HL, Lin WC, Lai PL, Hu RH, Yuan RH: Cytoplasmic CD24 expression is a novel prognostic factor in diffuse-type gastric adenocarcinoma. Ann Surg Oncol. 2007 Oct;14(10):2748-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/17680316ordin,*Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int. 2007 Nov;72(9):1095-102., URL: https://www.ncbi.nlm.nih.gov/pubmed/17700640ordin,*Schabath H, Runz S, Joumaa S, Altevogt P: CD24 affects CXCR4 Functional Application (FUNC)tion in pre-B lymphocytes and breast carcinoma cells. J Cell Sci. 2006 Jan 15;119(Pt 2):314-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16390867ordin,*Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J: CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system. J Immunol. 2001 May 1;166(9):5567-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/11313396ordin

NCBI and Uniprot Product Information

• NCBI GI #: 143811372
• NCBI GeneID: 100133941
• UniProt Accession #: P25063; Q16257; Q53XS0
• Molecular Weight: 80
• NCBI Official Full Name: Signal transducer CD24
• NCBI Official Synonym Full Names: CD24 molecule
• NCBI Official Symbol: CD24
• NCBI Official Synonym Symbols: CD24A
• NCBI Protein Information: signal transducer CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• UniProt Protein Name: Signal transducer CD24
• Protein Family: Signal transducer
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A
• UniProt Entry Name: CD24_HUMAN

NCBI Description

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Membrane protein, GPI anchor; Cell surface; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function: protein binding; signal transducer activity; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; cholesterol homeostasis; cell-cell adhesion; elevation of cytosolic calcium ion concentration; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; negative regulation of transforming growth factor-beta3 production; T cell costimulation; regulation of epithelial cell differentiation; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To