Mouse TLR4 Monoclonal Antibody | anti-TLR4 antibody

TLR4, Human, mAb HTA125

Cat. Num. AAA584009

• Reactivity: Reactivity: Human. Cross reactivity: Canine, Monkey
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: TLR4; Monoclonal Antibody; Human; mAb HTA125; CD284; ARMD10; anti-TLR4 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Monkey
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 55

• Applicable Applications for anti-TLR4 antibody: Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Westen Blot (WB)
• Application Notes: Application Notes: FC: 300000 cells/50ul were stained with 2 ug antibody for 30 minutes at 4 degree C; Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TLR4 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
• Immunogen: BALB/c mice were immunized with the Ba/F3 line expressing TLR4.
• Positive Control: Macrophages
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR4 antibody

Product Description: Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called ˜pathogen-associated molecular patterns. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NF degree B-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single alpha-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro'inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferon'beta) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2.

References

1. Shimazu, R et al; MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med 1999, 189: 1777
2. Tabeta, K et al; Toll-like receptors confer responsiveness to lipopolysaccharide from porphyromonas gingivalis in human gingival fibroblasts. Infect Immun 2000, 68: 3731
3. Akashi, S et al; Regulatory roles for CD14 and phosphatidylinositol in the signaling via Toll-like receptor 4-MD-2. Biochem Biophys Res Commun 2000, 268: 172
4. Wang, J et al; Involvement of CD14 and Toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 2402
5. Walton, K et al; Receptors involved in the oxidized 1-palmitoyl-2 arachidonoyl-sn-glycero-3- phosphorylcholine-mediated synthesis of interleukin-8. J Biol Chem 2003, 278: 29661
6. Triantafilou, M et al; Lateral diffusion of Toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane. J Cell Sci 2004, 117: 4007
7. Elner, S et al; TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression. Trans Am Ophthalmol Soc 2005, 103: 126
8. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124: 184
9. Brll, F et al; TLR2 activation is essential to induce a Th1 shift in human peripheral blood mononuclear cells by plant stanols and plant sterols. J Biol Chem 2010, 285: 2951

NCBI and Uniprot Product Information

• NCBI GI #: 260401829
• Molecular Weight: 6,356 Da
• NCBI Official Full Name: TLR4, partial
• UniProt Protein Name: TLR4
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR4
• UniProt Entry Name: D0EL75_BUBBU

Uniprot Description

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.