CLEC-2 Active Protein | CLEC1B active protein

CLEC-2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120607

• Gene Names: CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• Purity: >76% as determined by SDS-PAGE
• Synonyms: CLEC-2; CLEC-2 Protein; Human; Recombinant (His Tag); Human CLEC1B/CLEC2 Protein (His Tag); 1810061I13Rik Protein; CLEC2 Protein; CLEC2B Protein; PRO1384 Protein; QDED721 Protein; C-type lectin domain family 1; member B; CLEC1B active protein

• Host: HEK293 Cells
• Purity/Purification: >76% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln58-Pro229

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CLEC1B (NP_057593.3) extracellular domain (Gln 58-Pro 229) with a N-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human Podoplanin at 10ug/mL (100uL/well) can bind biotinylated human CLEC1B-His. The EC50 of biotinylated human CLEC1B-His is 0.71ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLEC1B active protein

Background: CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.

Product Categories/Family for CLEC1B active protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Suzuki-Inoue K, et al. (2007) Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells. J Biol Chem. 282(36):25993-6001.Watson AA, et al. (2007) The crystal structure and mutational binding analysis of the extracellular domain of the platelet-activating receptor CLEC-2. J Biol Chem. 282(5):3165-72.Chaipan C, et al. (2006) DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. J Virol. 80(18):8951-60.

NCBI and Uniprot Product Information

• NCBI GI #: 153252029
• NCBI GeneID: 51266
• NCBI Accession #: NP_057593.3
• NCBI GenBank Nucleotide #: NM_016509.3
• UniProt Accession #: Q9P126; Q6UWX7; Q8NHR6
• Molecular Weight: 23,096 Da
• NCBI Official Full Name: C-type lectin domain family 1 member B isoform a
• NCBI Official Synonym Full Names: C-type lectin domain family 1 member B
• NCBI Official Symbol: CLEC1B
• NCBI Official Synonym Symbols: CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• NCBI Protein Information: C-type lectin domain family 1 member B
• UniProt Protein Name: C-type lectin domain family 1 member B
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC1B
• UniProt Synonym Gene Names: CLEC2; CLEC-2
• UniProt Entry Name: CLC1B_HUMAN

NCBI Description

Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan 2011]

Uniprot Description

CLEC1B: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; defense response; platelet activation