Rabbit ENX-2 Polyclonal Antibody | anti-ENX-2 antibody

ENX-2 (E192) Polyclonal Antibody

Cat. Num. AAA3002182

• Gene Names: EZH1; KMT6B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ENX-2; Polyclonal Antibody; ENX-2 (E192) Polyclonal Antibody; Histone-lysine N-methyltransferase EZH1; ENX2; Enhancer of zeste homolog 1; EZH1; KIAA0388; anti-ENX-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ENX-2 (E192) polyclonal antibody detects endogenous levels of ENX-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 747

• Applicable Applications for anti-ENX-2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 162-208 of Human ENX-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ENX-2 (E192) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug) Lane6:H9C2 whole cell lysate (40ug) Lane7:3T3-L1 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ENX-2 (E192) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-ENX-2 antibody

In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG) proteins, which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. ENX-2 is another mammalian homolog of the Drosophila gene enhancer of zeste and contains one SET domain. The gene for human ENX-2 maps to chromosome 17q21. ENX-2 expression is ubiquitous in adult and fetal tissue, where it may aid in maintaining heterochromatin stability.

NCBI and Uniprot Product Information

• NCBI GI #: 19923202
• NCBI GeneID: 2145
• NCBI Accession #: NP_001982.2
• NCBI GenBank Nucleotide #: NP_001982.2
• UniProt Accession #: Q92800
• Molecular Weight: ~ 85kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase EZH1 isoform 1
• NCBI Official Synonym Full Names: enhancer of zeste 1 polycomb repressive complex 2 subunit
• NCBI Official Symbol: EZH1
• NCBI Official Synonym Symbols: KMT6B
• NCBI Protein Information: histone-lysine N-methyltransferase EZH1
• UniProt Protein Name: Histone-lysine N-methyltransferase EZH1
• UniProt Gene Name: EZH1
• UniProt Synonym Gene Names: KIAA0388
• UniProt Entry Name: EZH1_HUMAN

NCBI Description

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

Uniprot Description

EZH1: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Belongs to the histone-lysine methyltransferase family. EZ subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex

Molecular Function: chromatin binding; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: anatomical structure morphogenesis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter