Testing Data
Rabbit anti-Human, Mouse Dio-1 Polyclonal Antibody | anti-Dio-1 antibody
Dio-1 Antibody
Gene Names
Dido1; dido; DIO-1; Datf1; DATF-1; 6720461J16Rik; D130048F08Rik
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity purified
Synonyms
Dio-1; Polyclonal Antibody; Dio-1 Antibody; BYE1; C20orf158; DATF1; DIDO2; DIDO3; DIO-1; DIO1; DKFZp434P1115; FLJ11265; KIAA0333; MGC16140; dJ885L7.8; anti-Dio-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
0.2 mg/ml (varies by lot)
Sequence Length
614
Applicable Applications for anti-Dio-1 antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes the 62 kDa human and mouse Dio-1 in Western blot analysis. Jurkat cell lysate and mouse small intestine tissue lysate can be used as positive controls.
Immunogen
Synthetic peptide near the N-terminus of mouse Dio-1
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-Dio-1 antibody
Background: Apoptosis plays an important role in many cellular processes. Dio-1 (Death Inducer-O bliterator-1), a putative transcription factor, has been indicated to function in the onset of apoptosis. Dio-1 expression is upregulated when an apoptosis is detected. It translocates to the nucleus, and activates apoptosis during limb development. Dio-1 expression can be suppressed by caspase inhibitors and Bcl-2 expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129,171 Da
NCBI Official Full Name
death-inducer obliterator 1 Dido1
NCBI Official Synonym Full Names
death inducer-obliterator 1
NCBI Official Symbol
Dido1
NCBI Official Synonym Symbols
dido; DIO-1; Datf1; DATF-1; 6720461J16Rik; D130048F08Rik
NCBI Protein Information
death-inducer obliterator 1
UniProt Protein Name
Death-inducer obliterator 1
UniProt Gene Name
Dido1
UniProt Synonym Gene Names
Datf1; Dio1; DIO-1; DATF-1
UniProt Entry Name
DIDO1_MOUSE
NCBI Description
This gene encodes a transcription factor involved in apoptosis. The encoded protein functions in cell cycle progression and plays a role in chromosomal stability. This protein regulates the self-renewal of embryonic stem cells. Disruption of this gene in mice causes symptoms similar to myelodysplastic/myeloproliferative diseases in humans. Mice lacking this gene show severely reduced fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Uniprot Description
DATF1: Putative transcription factor, weakly pro-apoptotic when overexpressed. Tumor suppressor. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor
Cellular Component: cytoskeleton; cytoplasm; nucleus
Molecular Function: zinc ion binding; metal ion binding
Biological Process: transcription, DNA-dependent; apoptosis
Research Articles on Dio-1
Similar Products
Product Notes
The Dio-1 dido1 (Catalog #AAA840813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dio-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dio-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes the 62 kDa human and mouse Dio-1 in Western blot analysis. Jurkat cell lysate and mouse small intestine tissue lysate can be used as positive controls. Researchers should empirically determine the suitability of the Dio-1 dido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dio-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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