Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CD45R antibody (MBS488608) RA3-6B2 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (MBS488608) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488608, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse CD45R Monoclonal Antibody | anti-CD45R antibody
Anti-CD45R [RA3-6B2]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD45R; Monoclonal Antibody; Anti-CD45R [RA3-6B2]; B220; CD45; CD45 antigen; Cluster designation 45R; L-CA; Leukocyte common antigen; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase; receptor type; C; c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; anti-CD45R antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, kappa
Clone Number
RA3-6B2
Specificity
This antibody is specific for murine CD45R antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R mAb clone RA3-6B2 specifically recognizes the exon A-restricted isoform of mouse CD45.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD45R antibody
Flow Cytometry (FC/FACS), WV, Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to understand the role of PD-1 in maintaining islet-specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades (Svedova et al, 2016), to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli (Brandstätter et al, 2016), and to examine the role of FBXW7 in suppression of cancer metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015).
PMID
12077275
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CD45R antibody (MBS488608) RA3-6B2 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (MBS488608) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488608, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CD45R antibody (MBS488608) RA3-6B2 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (MBS488608) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488608, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD45R antibody RA3-6B2 (MBS488608). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of RA3-6B2 (MBS488608, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD45R antibody RA3-6B2 (MBS488608). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of RA3-6B2 (MBS488608, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-CD45R antibody
Recombinant monoclonal antibody to CD45R. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RA3-6B2.
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Product Categories/Family for anti-CD45R antibody
References
Stephen Hurst et al. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1):443-53.
Note on publication: Describe the use of this antibody for FACS analysis to demonstrate the function of the novel cytokine IL-25 in the lung.
Note on publication: Describe the use of this antibody for FACS analysis to demonstrate the function of the novel cytokine IL-25 in the lung.
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
Ptprc
UniProt Synonym Gene Names
Ly-5
UniProt Entry Name
PTPRC_MOUSE
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Product Notes
The CD45R ptprc (Catalog #AAA488609) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD45R [RA3-6B2] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), WV, Immunohistochemistry (IHC), Immunofluorescence (IF). This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to understand the role of PD-1 in maintaining islet-specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades (Svedova et al, 2016), to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli (Brandstätter et al, 2016), and to examine the role of FBXW7 in suppression of cancer metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015). Researchers should empirically determine the suitability of the CD45R ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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