Mouse anti-Human TCR Vdelta2 Monoclonal Antibody | anti-TCRVdelta2 antibody

Anti-Hu TCR Vdelta2 Purified

Cat. Num. AAA1801836

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by protein-A affinity chromatography
• Synonyms: TCR Vdelta2; Monoclonal Antibody; Anti-Hu TCR Vdelta2 Purified; TCRVD2; anti-TCRVdelta2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: B6
• Specificity: The mouse monoclonal antibody B6 recognizes an extracellular epitope of human TCR Vdelta2, which is expressed on the majority of gamma/delta T cells.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TCRVdelta2 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml
• Immunogen: human recombinant TCR Vdelta2
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 ug/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human TCR Vdelta2 positive lymphocytes (red-filled) from human TCR Vdelta2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 ug/ml, GAM APC).)

Related Product Information for anti-TCRVdelta2 antibody

TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.

References

*Siegers GM, Felizardo TC, Mathieson AM, Kosaka Y, Wang XH, Medin JA, Keating A: Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS One. 2011 Feb 3;6(2):e16700., URL: https://pubmed.ncbi.nlm.nih.gov/21304898/,*Hunter S, Willcox CR, Davey MS, Kasatskaya SA, Jeffery HC, Chudakov DM, Oo YH, Willcox BE: Human liver infiltrating gammadelta T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018 Sep;69(3):654-665., URL: https://pubmed.ncbi.nlm.nih.gov/29758330/,*Ogongo P, Steyn AJ, Karim F, Kaylesh J Dullabh KJ, Awala I, Madansein R, Leslie A, Behar SM: Differential skewing of donor-unrestricted and gammadelta T cell repertoires in tuberculosis-infected human lungs. J Clin Invest. 2020 Jan 2;130(1):214-230., URL: https://pubmed.ncbi.nlm.nih.gov/31763997/,*Li H, Luo K, Pauza CD: TNF-alpha is a positive regulatory factor for human Vgamma2 Vdelta2 T cells. J Immunol. 2008 Nov 15;181(10):7131-7, URL: https://pubmed.ncbi.nlm.nih.gov/18981134/,*Fisher JP, Yan M, Heuijerjans J, Carter L, Abolhassani A, Frosch J, Wallace R, Flutter B, Capsomidis A, Hubank M, Klein N, Callard R, Gustafsson K, Anderson J: Neuroblastoma killing properties of Vdelta2 and Vdelta2-negative gammadeltaT cells following expansion by artificial antigen-presenting cells. Clin Cancer Res. 2014 Nov 15;20(22):5720-32., URL: https://pubmed.ncbi.nlm.nih.gov/24893631/,*Davey MS, Willcox CR, Hunter S, KasatskayaSA, Remmerswaal EB, Salim M, Mohammed F, Bemelman FJ, Chudakov DM, Oo YH, Willcox BE: The human Vdelta2 + T-cell compartment comprises distinct innate-like Vgamma9 + and adaptive Vgamma9 - subsets. Nat Commun. 2018 May 2;9(1):1760., URL: https://pubmed.ncbi.nlm.nih.gov/29720665/